DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems
نویسندگان
چکیده
منابع مشابه
Culture and Assay Systems Used for 3D Cell Culture
Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...
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Cell-based assays are one of the most important steps to select huge amount of drug candidates in drug discovery. To get more credible assay results, cell culture in the form of microscale environment and three-dimension has been exploited by microfluidic hydrodynamic focusing. However, the method still needs an enhanced reliability of scaffold formation and fast cell immobilization in a microc...
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Three-dimensional (3D) cultures are becoming increasingly popular due to their ability to mimic tissuelike structures more effectively than monolayer cultures. In cancer research, the natural tumor characteristics and architecture are more closely mimicked by 3D cell models. Thus, 3D cell cultures are more promising and suitable models, particularly for in vitro drug screening to predict in viv...
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This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choi...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-71265-7